A Paleolithic diet confers higher insulin sensitivity, lower C-reactive protein and lower blood pressure than a cereal-based diet in domestic pigs
- Tommy Jönsson1Email author,
- Bo Ahrén1,
- Giovanni Pacini2,
- Frank Sundler3,
- Nils Wierup4,
- Stig Steen5,
- Trygve Sjöberg5,
- Martin Ugander6,
- Johan Frostegård7,
- Leif Göransson8 and
- Staffan Lindeberg1
© Jönsson et al; licensee BioMed Central Ltd. 2006
Received: 17 July 2006
Accepted: 02 November 2006
Published: 02 November 2006
A Paleolithic diet has been suggested to be more in concordance with human evolutionary legacy than a cereal based diet. This might explain the lower incidence among hunter-gatherers of diseases of affluence such as type 2 diabetes, obesity and cardiovascular disease. The aim of this study was to experimentally study the long-term effect of a Paleolithic diet on risk factors for these diseases in domestic pigs. We examined glucose tolerance, post-challenge insulin response, plasma C-reactive protein and blood pressure after 15 months on Paleolithic diet in comparison with a cereal based swine feed.
Upon weaning twenty-four piglets were randomly allocated either to cereal based swine feed (Cereal group) or cereal free Paleolithic diet consisting of vegetables, fruit, meat and a small amount of tubers (Paleolithic group). At 17 months of age an intravenous glucose tolerance test was performed and pancreas specimens were collected for immunohistochemistry. Group comparisons of continuous variables were made by use of the t-test. P < 0.05 was chosen for statistical significance. Simple and multivariate correlations were evaluated by use of linear regression analysis.
At the end of the study the Paleolithic group weighed 22% less and had 43% lower subcutaneous fat thickness at mid sternum. No significant difference was seen in fasting glucose between groups. Dynamic insulin sensitivity was significantly higher (p = 0.004) and the insulin response was significantly lower in the Paleolithic group (p = 0.001). The geometric mean of C-reactive protein was 82% lower (p = 0.0007) and intra-arterial diastolic blood pressure was 13% lower in the Paleolithic group (p = 0.007). In evaluations of multivariate correlations, diet emerged as the strongest explanatory variable for the variations in dynamic insulin sensitivity, insulin response, C-reactive protein and diastolic blood pressure when compared to other relevant variables such as weight and subcutaneous fat thickness at mid sternum. There was no obvious immunohistochemical difference in pancreatic islets between the groups, but leukocytes were clearly more frequent in sampled pancreas from the Cereal group.
This study in domestic pigs suggests that a Paleolithic diet conferred higher insulin sensitivity, lower C-reactive protein and lower blood pressure when compared to a cereal based diet.
Our pre-agricultural, hunter-gatherer human ancestors during the Paleolithic period (the old stone age; 2.5 million – 10,000 years BP) had a diet based on vegetables, fruit, nuts, roots, meat, organ meats and insects . This Paleolithic diet has been suggested to be more in concordance with our evolutionary legacy than a diet based on products associated with agriculture during the Neolithic period (10,000 years BP – present time) such as cereals and milk [2, 3]. A diet in less concordance with our evolutionary legacy might confer diseases due to insufficient adaptation  possibly explaining the reported lower incidence of diseases of affluence , such as type 2 diabetes, obesity and cardiovascular disease among Non-Western ethnic groups with hunter-gatherer lifestyles and diets [6, 7]. However, the mechanisms behind this reported lower incidence of diseases of affluence are largely unknown, and commonly discussed dietary factors such as fat (amount and quality) and (cereal) fiber may be of limited importance [8–10]. A possible mechanism might be the potential of Paleolithic diet to reduce risk factors for diseases of affluence, such as disturbed glucose homeostasis, insulin resistance and hypertension . Food restriction induced by the satiating properties of a Paleolithic diet  may also play a role. Food restriction, sometimes referred to as dietary restriction or caloric restriction , of 20–40% of dietary energy compared to free feeding (ad libitum) has been shown to reduce the incidence of several diseases of affluence in mammals including non-human primates, and possibly humans [12, 13]. Interestingly, Non-Western ethnic groups with hunter-gatherer lifestyles and diets stay lean and apparently reap health benefits similar to those induced by food restriction despite ad libitum availability of food .
The aim of this study was to determine experimentally whether a Paleolithic diet on a long-term basis affects risk factors for diseases of affluence in a prospective and randomized setting. This was assessed in domestic swine by examining glucose tolerance, post-challenge insulin response, plasma C-reactive protein and blood pressure after 15 months on a Paleolithic diet in comparison with a cereal based swine feed supplemented with rapeseed oil.
Animals and diet
Provisions during last three months in study
Cereal swine feed
At age 17 months all animals received premedication in the stable with intramuscular ketamine (Ketalar, Parke-Davis, Morris Plains, NJ), 7.5 mg/kg body weight and xylasin (Rompun, Bayer, Gothenburg, Sweden) 0.125 mg/kg. When sedated an intravenous needle was inserted in an ear vein and thiopental (Pentothal, Abbot, North Chicago, Il) 0.5 mg/kg and atropine (Atropine, Kabi Pharmacia, Uppsala, Sweden) 0.02 mg/kg were given. During the experiment, anesthesia was maintained using propofol (Diprivan, AstraZeneca, Södertälje, Sweden) 9–15 mg/kg/h. Fentanyl (Leptanal, Janssen-Cilag, Sollentuna, Sweden) 0.02 μg/kg and atracurium (Tracrium, Epipharm, Gnesta, Sweden) 0.2–0.5 mg/kg were given intermittently for pain management and muscular relaxation, respectively. The animals were ventilated with a Siemens Servo ventilator 300 (Siemens-Elema AB, Solna, Sweden). A volume-controlled, pressure-regulated ventilation of 14–20 L/min (15 breaths/min; positive end-expiratory pressure [PEEP], 5 cm H2O; inspired oxygen fraction, 0.5; max inspiratory pressure, 30 cm H2O) was used.
An intravenous glucose tolerance test (IVGTT) was performed in 20 pigs which had been fasted for at least 6 hours. The pigs were kept in a supine position during the IVGTT, and hemodynamic values and body temperature (measured by naso-pharyngeal probe) were recorded using a data acquisition system (Testpoint; Capital Equipment Corp, Billerica, MA). A catheter was introduced into the external jugular vein and threaded into the superior vena cava for both glucose administration and blood sampling. At -5 min and -30 seconds before glucose administration, 2.5-ml blood samples were collected. At time 0, glucose (0.5 g/kg body weight) was infused over 20–30 seconds. Thereafter, 2.5 ml blood samples were drawn at 2, 3, 4, 5, 6, 8, 10, 12, 14, 16, 18, 19, 22, 25, 30, 35, 40, 45, 50, 55, 60, 75, 90, 105 and 120 min. An equivalent amount of sterile saline solution was used to flush the catheter line. The tubes were centrifuged, and plasma was stored at -20°C for analysis of insulin and glucose concentrations using standard methods. Body length was measured from nose to end of buttocks. Subcutaneous fat thickness was measured with a ruler at mid sternum after the thorax was opened. For immunohistochemistry of islet hormones and islet morphology, specimens were collected from mid- and tail portions of the pancreas from 5 pigs in each group. Two specimens were collected from each site. One specimen was fixed overnight in buffered formaldehyde (4 %), dehydrated in graded ethanols, and embedded in paraffin. The other specimen was fixed in Stefanini's solution (2 % paraformaldehyde and 0.2 % picric acid in 0.1 M phosphate buffer, pH 7.2), rinsed thoroughly in Tyrode solution containing 10 % sucrose, and frozen on dry ice. Sections from paraffin-embedded specimens, and cryosections were then processed for indirect immunofluorescence using well characterised islet hormone antisera as primary antibodies, and FITC-labelled second antibodies. For details on the antibodies used, and the immunostaining protocol, see e.g. Wierup et al 2002 .
Data analysis and statistics
Final clinical characteristics (mean ± standard deviation)
Paleolithic group (n = 11)
Cereal group (n = 12)
129 ± 16
166 ± 28
159 ± 6
170 ± 9
Subcutaneous fat (cm)
1.9 ± 0.4
3.3 ± 0.9
Body temperature (°C)
37.7 ± 1.5
37.6 ± 0.5
Systolic Blood Pressure (mm Hg)
140 ± 18
150 ± 9
Diastolic Blood Pressure (mm Hg)
108 ± 12
123 ± 12
Final glucometabolic characteristics (mean ± standard deviation)
Paleolithic group (n = 9)
Cereal group (n = 9)
5.6 ± 1.4
6.0 ± 1.9
AUC1 glucose 0–120 min (mmol/l min)
1076 ± 113
1199 ± 212
0.58 ± 0.12
0.67 ± 0.17
8.3 ± 4.6
9.2 ± 4.6
0.66 ± 0.15
0.70 ± 0.36
Dynamic insulin sensitivity (%min-1/(pmol/l))
2.35 ± 0.76
1.41 ± 0.39
AUC1 insulin 0–120 min (pmol/l min)
2613 ± 863
4 973 ± 1476
AUC1 insulin 0–120 min stimulated secretion (pmol/l min)
1620 ± 1074
3872 ± 1112
Acute insulin response 2–4 min (pmol/l)
14.9 ± 8.3
15.8 ± 10.7
Evaluations of bivariate correlation showed that weight was highly correlated with subcutaneous fat thickness (adjusted R2 = 0.77, p < 0.0001). Evaluations of multivariate correlation with forward stepwise linear regression analysis was performed in order to study variables independently explaining the variation of dynamic insulin sensitivity, AUCins0–120, log C-reactive protein and diastolic blood pressure, respectively. In each of these four regression models diet was entered as independent variable and evaluated with each one of the remaining relevant study variables as the second independent variable. Diet emerged as the strongest explanatory variable for variations in dynamic insulin sensitivity, AUCins0–120, log C-reactive protein and diastolic blood pressure.
This study showed highly beneficial effects of a Paleolithic diet on risk factors for diseases of affluence when compared to cereal based swine feed. A strength of the study is that the model used is one of the best non-primate models for human disorders, the domestic pig . The present study indicated no difference in glucose disappearance during IVGTT between the groups. During IVGTT plasma insulin increased successively between 30 and 80 min in both groups, possibly due to propofol anesthesia [21, 22], and this precluded the use of the minimal model of glucose disappearance [16, 17]. However, other indices of dynamic insulin sensitivity clearly showed higher insulin sensitivity in the Paleolithic group, which also exhibited a significantly lower insulin response to injected glucose. It is worth noting that the difference in insulin response between groups during IVGTT was observed in the late phase of insulin secretion. Evaluation of multivariate correlation showed that the beneficial effect of Paleolithic diet on insulin sensitivity and insulin response was independent of all other relevant study variables such as body weight, subcutaneous fat thickness and body temperature. A Paleolithic diet thus conferred higher insulin sensitivity, which is central to the prevention of cardiovascular disorders , and consistent with our finding in humans . Furthermore, contrary to our survey in humans , this finding of higher insulin sensitivity was associated with no significant difference between groups in fasting levels of insulin and glucose, indicating that the Paleolithic diet affected insulin action mainly at the level of muscle and adipose tissues rather than the liver .
The Paleolithic group also showed significantly lower levels of C-reactive protein, a physiologic marker of subclinical inflammation, which has been shown to be associated with insulin resistance and cardiovascular disease [23, 25]. Elevated levels of C-reactive protein has been suggested to reflect overproduction by expanded adipose tissue mass . However, evaluation of multivariate correlation showed that the beneficial effect of Paleolithic diet on C-reactive protein was independent of all other relevant study variables including measures of obesity such as body weight and subcutaneous fat thickness. Interestingly, immunohistochemical analysis suggested a diffuse and low-grade pancreatic inflammation in the cereal group, as evidenced by clearly more frequent leukocytes scattered throughout their exocrine pancreatic parenchyma or clustered around pancreatic ducts and blood vessels. This finding offers a novel approach in the research on the association between inflammation and type 2 diabetes . Immunohistochemical analysis also showed that the cereal group could compensate for both pancreatic inflammation and increased need of insulin due to lower insulin sensitivity, without noticeable differences in pancreatic islets between groups.
The shorter length in the Paleolithic group is not unexpected if we consider length in pigs as a correlate of height in humans. Available evidence suggests that hunter-gatherers and similar ethnic groups are shorter than Western populations, and a positive relationship between height and cardiovascular disease has been noted in international comparisons . Insulin resistance and hyperinsulinemia can hypothetically promote growth by way of insulin-like growth factors .
In addition to these significant results, intra-arterial diastolic blood pressure was significantly lower in the Paleolithic group, and this beneficial effect of Paleolithic diet was also independent of all other relevant study variables. The present study thus provides experimental evidence for beneficial effects of a Paleolithic diet on risk factors for diseases of affluence, which might account for their low incidence reported among Non-Western ethnic groups with hunter-gatherer lifestyles and diets [6, 7].
The mechanisms behind these beneficial effects on risk factors are largely unknown. In evaluations of multivariate correlation, diet emerged as a stronger explanatory variable than any other relevant variable for variations in dynamic insulin sensitivity, AUCins0–120, log C-reactive protein and diastolic blood pressure. The observed effects on risk factors thus seem to be primarily caused by diet. However, it is conceivable that this dietary effect on risk factors is a result of differences in other variables induced by dietary assignment. An important finding in this regard is the divergence of the weight curves between the two groups after 3 months of feeding. The Paleolithic group was thus lighter and had a lower energy intake at the end of the study than the Cereal group, despite a threefold larger ration by weight. The diverging weight curves could be interpreted in several ways. They could be interpreted as food restriction in the Paleolithic group, which could explain the observed effects on risk factors . Neither group was fed their respective diet ad libitum but was rather allocated rations on a group basis judged sufficient to achieve healthy animals by an experienced experimental pig farmer. This procedure was chosen due to concerns about the unfamiliar Paleolithic diet as swine feed, and the fact that feeding ad libitum is not the custom in Swedish swine production. The diverging weight curves between the groups could thus possibly be caused by differences in subjectively allocated rations (e.g. difference in energy intake), which could lead to food restriction in the Paleolithic group. However, food restriction typically lowers mean body temperature by 1–2°C , and we found no significant difference in mean body temperature between the two groups, indicating that there was not a substantial food restriction in the Paleolithic group as compared to the Cereal group. In fact, the mean weight in the Paleolithic group is well within the normal range of pigs . The diverging weight curves are thus probably not caused by food restriction in the Paleolithic group but instead could be interpreted as obesity in the Cereal group in analogy with human classification of individual weight based on statistical health effects . The significant difference in subcutaneous fat thickness between groups and the high correlation between weight and subcutaneous fat thickness supports this notion. Obesity in humans is associated with increased insulin resistance, high blood pressure and high levels of C-reactive protein , and could explain the observed effects on risk factors. The difference between the two diets regarding obesity promotion could still be due to differences in subjectively allocated rations, but could alternatively be due to properties of a cereal based swine feed which possibly disturb the regulation of satiation , satiating properties of a Paleolithic diet  and differing effects between the diets on energy metabolism . The satiating properties of a Paleolithic diet could be due to differences in macronutrient diet composition, such as the low protein content of cereals in the Cereal group diet. The Paleolithic group thus ate relatively more protein and less carbohydrates, which through satiating, thermogenic and other properties could account for the results of the study [32, 33], although results from studies on the association between protein intake and diseases of affluence have been contradictory . In future studies of Paleolithic diets it would be valuable to match diets for macronutrient composition. The latter alternative explanations for the diverging weight curves is supported by our epidemiological findings in humans from Kitava, Papua New Guinea, where a non-western lifestyle has been connected to leanness despite food being available ad libitum . The diverging weight curves would then suggest that the beneficial effects on risk factors could be due to differences between diets regarding obesity promotion. Alternatively, obesity could be a marker for other effecting mechanisms, such as leptin resistance, and the diverging weight curves would then suggest that the beneficial effects on risk factors could be due to differences between diets regarding promotion of leptin resistance .
In conclusion, this study in domestic pigs suggests that a Paleolithic diet as compared to a cereal based diet conferred higher insulin sensitivity, lower C-reactive protein and lower blood pressure. The results suggest that pigs, as previously suggested for humans , are not specifically adapted through evolution to a diet incorporating large amounts of cereals, which could confer diseases of affluence as a sign of insufficient evolutionary adaptation .
The authors are grateful to Lilian Bengtsson, Jun Su and Doris Persson for technical assistance. The study was supported by grants from the Swedish Research Council (6834 and 4499), Swedish Diabetes Association, Albert Påhlsson Foundation, Novo Nordic Foundation, Region Skåne and the Faculty of Medicine, Lund University.
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