Dietary Phytoestrogens Influence on Uncoupling Protein-1 (UCP-1) mRNA Levels in Brown Adipose Tissue (BAT) from males fed either a phytoestrogen-rich (Phyto-600) or a phytoestrogen-free (Phyto-free) diet. For each sample, the optical density (O.D.) signal ratio of BAT UCP-1 mRNA abundance to ribosomal 18S RNA intensity was determined. The results represent the mean O.D. values and S.E.M. of 8 independent samples by diet treatment of at least two runs of the RT-PCR protocol. Males fed the Phyto-600 diet expressed significantly higher BAT UCP-1 mRNA levels (* p < 0.05) compared to Phyto-free values.