Table 2 Concentrations of oxidative stress parameters and relative mRNA expression of TNF-α, AKT1, Fas and IFN-γ induced in the different groups
Parameters | Control group | CCL4 | Me | CCL4+Me | Fo | CCL4+Fo | CCL4+Me + Fo |
|---|---|---|---|---|---|---|---|
Blood Hydroperoxide (mg/100 ml) | 24 ± 5.0 | 44.5* ± 6.2 | 21.5 ± 4.3 | 32.1* ± 4.2 | 21.8 ± 3.0 | 30.6 ± 6.3 | 27 ± 5.0 |
Hepatic MDA (nmol/mg protein) | 3 ± 0.7 | 5.6* ± 0.9 | 2.1 ± 0.8 | 4* ± 0.7 | 2.4 ± 0.7 | 4.4* ± 0.9 | 2.1 ± 0.6 |
Hepatic GSH (μmol/g liver tissue) | 12.2 ± 1.8 | 5* ± 1.8 | 25.7* ± 7.8 | 14.7 ± 5.0 | 28.6* ± 5.8 | 21.7* ± 5.0 | 21.2* ± 5.1 |
Catalase (Unit/mg protein) | 14.4 ± 1.8 | 7.9* ± 1.0 | 16.3 ± 1.8 | 11.5 ± 2.8 | 16 ± 2.7 | 10* ± 0.9 | 14 ± 1.5 |
Relative expression of TNF-α mRNA | 1.8 ± 0.8 | 5.6* ± 0.9 | 1.6 ± 0.5 | 3.3* ± 0.7 | 2.1 ± 0.8 | 3.2* ± 0.7 | 1.7 ± 0.5 |
Relative expression of Akt1 mRNA | 5.7 ± 1.1 | 1.8* ± 0.5 | 15.2* ± 2.7 | 5.0 ± 1.3 | 17* ± 1.8 | 5.0 ± 1.6 | 7.0 ± 1.8 |
Relative expression of Fas mRNA | 0.9 ± 0.3 | 10.1* ± 0.9 | 0.8 ± 0.45 | 8.2* ± 1.2 | 0.78 ± 0.2 | 5.5* ± 0.2 | 2.5* ± 0.7 |
Relative expression of IFN- γ mRNA | 18.5 ± 7.2 | 7.0* ± 2.2 | 17.5 ± 6.0 | 8.8* ± 0.9 | 34.2* ± 4.4 | 14.6 ± 2.6 | 20.2 ± 3.0 |