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Table 3 Morphologic parameters, food intake and plasma lipid concentrations of mice

From: n-3 PUFA added to high-fat diets affect differently adiposity and inflammation when carried by phospholipids or triacylglycerols in mice

Morphologic parameters Mice groups according to dietary lipids
LF HF HF-ω3PL HF-ω3TG
Biometric data     
Initial Body weight (g) 24.1 ± 0.4 24.2 ± 0.3 23.9 ± 0.3 24.5 ± 0.3
Body weight gain (g) 4.1 ± 0.1 4.2 ± 0.5 3.1 ± 0.1*$ 3.7 ± 0.3
Energy Intake (kJ/mouse/d) 39.1 ± 0.9 68.9 ± 0.3$ 60.2 ± 1.3$ 59.8 ± 1.3$
Liver weight (g) 1.39 ± 0.03 1.36 ± 0.03 1.21 ± 0.04*$ 1.27 ± 0.04*
WAT weight (g) 0.84 ± 0.05 1.03 ± 0.12 0.72 ± 0.05* 0.93 ± 0.11
Gastrocnemius (g) 0.16 ± 0.0 0.16 ± 0.0 0.16 ± 0.0 0.15 ± 0.0
Plasma lipids     
TAG (mM) 0.89 ± 0.06 0.66 ± 0.09$ 0.64 ± 0.04$ 0.57 ± 0.04$
NEFA (mM) 0.62 ± 0.5 0.35 ± 0.02$ 0.27 ± 0.01$ 0.37 ± 0.02$
Glucose (mmol/L) 9.1 ± 0.8 8.6 ± 0.5 9.4 ± 0.9 9.2 ± 0.7
Plasma insulin (pmol/L) 37.0 ± 6.9 36.8 ± 3.0 24.5 ± 3.0 26.5 ± 4.8
Liver lipids (mg total fatty acids/g tissue) 30.3 ± 2.9 34.0 ± 3.0 30.3 ± 0.6 33.1 ± 3.7
Lipid peroxidation markers in plasma (nM)     
4-HHE 23.3 ± 13.5 103.7 ± 36.5 88.8 ± 32.0 128.0 ± 37.5
4-HNE 4.9 ± 0.3* 13.4 ± 0.5 5.9 ± 1.1* 8.6 ± 0.3*
  1. (*P < 0.05 vs HF); ($P < 0.05 vs LF), ANOVA followed by Fisher test.
  2. Data are mean ± SEM for n = 8 per group. Abbreviations: WAT, white adipose tissue; TAG, plasma triacyglycerols; NEFA, non-esterified fatty acids; 4-HHE, 4-hydroxy-2-hexenal; 4-HNE, 4-hydroxy-2-nonenal.
  3. For 4-hydroxy-2-alkenals analysis, 3 pools of 300 μL obtained from 3 mice (100 μL per mice) were used for each group (n = 3).