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Figure 2 | Nutrition & Metabolism

Figure 2

From: Supplementation of carnitine leads to an activation of the IGF-1/PI3K/Akt signalling pathway and down regulates the E3 ligase MuRF1 in skeletal muscle of rats

Figure 2

Relative protein levels of ubiquitin-protein conjugates in M. quadriceps femoris of rats fed either a control diet (0 mg carnitine/ kg diet; Control) or a diet supplemented with 1250 mg carnitine/ kg diet (Carnitine) . (A) A representative immunoblot specific to ubiquitin is shown for three animals per group; immunoblots for the other animals revealed similar results. Reversible staining of nitrocellulose membranes with Ponceau S revealed equal loading of protein. (B) Bars represent data from densitometric analysis and represent means ± SD (n = 6/group); bars are expressed relative to the protein level of the control group (= 1.00). * indicates a significant difference to the control group (P < 0.05).

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