Effect of CR and spice compounds on total number of spleen cells and proportion of CD4+T cells. Isolated splenocytes were counted under microscope for total spleen cells (A) and stained with fluorescence-conjugated anti-CD4 antibody, followed by flow cytometry to determine the splenic CD4+ T cell levels (B). Values are means ± SEM, n = 9 mice/group. Means without a common letter differ at P < 0.05. HFD: high fat diet, CR: caloric restriction, C: curcumin, P: piperine.