Analyzing the effect of cellular treatments on 13C
glucose uptake and GLUT-1 expression. Continuous (or chronic) cellular exposure to 100 μM ARG for 7 days in the presence or absence of 30 μM L-NAME or 30 μM CMP-C decreased 13C6 glucose uptake from the medium (A) and GLUT-1 expression (B). Inhibition of cellular accumulation of glucose via gluconeogenesis improved 13C6 glucose and GLUT-1 expression. n = 6. Acute (or short-term) treatment represents 2 h exposure to cells with various agents. *, represents significant variation between control and treatment groups at p < 0.05. §, represents variation in treatment groups from cells treated with 100 μM ARG, at p < 0.05.