Figure 4From: AMP-activated protein kinase regulates L-arginine mediated cellular responsesMeasurement of cellular AMPK activity and expression. AMPK expression decreased only during continuous (or chronic) exposure to 100 μM ARG in the presence or absence of 30 μM CMP-C or L-NAME for 7 days (A). Continuous co-incubation of cells with 100 μM ARG and 30 μM CDB for 7 days increased AMPK expression. Cells incubated short-term (or acutely) with 100 μM ARG for 2 h or when continuous co-treated with 100 μM ARG and 30 μM CDB showed increase in their AMPK activity (B). Co-incubation of cells with 100 μM ARG and either 30 μM CMP-C or L-NAME suppressed AMPK activity under all treatment conditions. n = 6. *, represents significant variation between control and treatment groups at p < 0.05. §, represents variation in treatment groups from cells treated with 100 μM ARG, at p < 0.05.Back to article page