Table 1 Characteristics of primers used for qPCR
Gene symbol | Primer sequence (forward, reverse; from 5′ to 3′) | NCBI GeneBank | Product size (bp) | Slope | R2# | Efficiency* |
|---|---|---|---|---|---|---|
(HUGO) | ||||||
ACADL | AAGGATTTATTAAGGGCAAGAAGC | NM_012819 | 380 bp | -3.88 | 0.998 | 1.81 |
GGAAGCGGAGGCGGAGTC | ||||||
ACADM | CAAGAGAGCCTGGGAACTTG | NM_016986 | 154 bp | -3.38 | 0.999 | 1.98 |
CCCCAAAGAATTTGCTTCAA | ||||||
ATP5B | GCACCGTCAGAACTATTGCT | NM_134364 | 203 bp | -3.59 | 0.999 | 1.90 |
GAATTCAGGAGCCTCAGCAT | ||||||
CANX | CCAGATGCAGATCTGAAGAC | NM_172008 | 175 bp | -2.75 | 0.999 | 2.31 |
CTGGGTCCTCAATTTCACGT | ||||||
CD36 | TCGTATGGTGTGCTGGACAT | NM_031561 | 358 bp | -3.28 | 0.996 | 2.02 |
GGCCCAGGAGCTTTATTTTC | ||||||
CPT1B | GCAAACTGGACCGAGAAGAG | NM_013200 | 180 bp | -3.32 | 0.988 | 2.00 |
CCTTGAAGAAGCGACCTTTG | ||||||
FABP3 | ACCATCCACTGCCGTCTTAC | NM_013177 | 310 bp | -3.20 | 0.957 | 2.05 |
CCCCGATGCGTAGGTATTCT | ||||||
HK2 | GATGGAATCGAGAAGGCCTA, | NM_012735 | 220 bp | -3.63 | 1.000 | 1.89 |
GTTTCTTGTAGACGGAGCCA | ||||||
LPL | GAGATTTCTCTGTATGGCACA | NM_012598 | 276 bp | -3.34 | 0.992 | 1.99 |
CTGCAGATGAGAAACTTTCTC | ||||||
MDH1 | CAGACAAAGAAGAGGTTGCC, | NM_033235 | 206 bp | -3.40 | 0.994 | 1.97 |
CGTCAGGCAGTTTGTATTGG | ||||||
MYH1 | GCAGACTCTCCCACTGGGCTG | NM_001135158 | 83 bp | -3.16 | 0.953 | 2.07 |
GAGCAGCCTCCCCGAAAACGG | ||||||
MYH2 | GCTGATCGAAATGCTGCTGA | NM_001135157 | 124 bp | -3.38 | 0.990 | 1.98 |
GTCAATAGCACTATCCGTGG | ||||||
MYH4 | CCAGTCCATCCTGATTACTG | NM_019325 | 74 bp | -3.48 | 0.988 | 1.94 |
CAAAGTACTGGATGACACGC | ||||||
MYH7 | ATTGCCGAGTCCCAGGTCAACA | NM_017240 | 127 bp | -3.24 | 0.944 | 2.03 |
GCTCCAGGTCTCAGGGCTTCAC | ||||||
PFKM | TCCTGGTTGGCTCAATCGAC | NM_031715 | 297 bp | -3.75 | 0.998 | 1.85 |
TGTTGAGACGAGAACCACGG | ||||||
PKM | ACCTGGGCATTGAGATTCCG | NM_053297 | 314 bp | -3.69 | 0.997 | 1.87 |
TCGCGCAAGCTCTTCAAACA | ||||||
PPARD | GCAGAGCTATGACCAGGCCTGCA | NM_013141 | 151 bp | -3.29 | 0.990 | 2.01 |
GTGCTCTGGTCCCCCGTTGA | ||||||
PPARGC1A | CTCTTTGCCCAGATCTTCCT | NM_031347 | 145 bp | -3.93 | 0.999 | 1.80 |
ATGTTCGCGGGCTCATTGTT | ||||||
PPARGC1B | CATATAAGCCCATGGAGGAG | NM_176075 | 476 bp | -3.25 | 0.978 | 2.03 |
CAGCCCAAAGTGCTTTGTGA | ||||||
RPL13 | CTTAAATTGGCCACGCAGCT | XR_086310 | 198 bp | -3.48 | 0.998 | 1.94 |
CTTCTCAACGTCTTGCTCTG | ||||||
SDHA | TGGACCTTGTCGTCTTTGG | NM_130428 | 88 bp | -3.90 | 0.997 | 1.80 |
TTTGCCTTAATCGGAGGAAC | ||||||
SLC2A4 | GAGTTATGTGTCCATCGTGG | NM_012751 | 187 bp | -2.59 | 0.953 | 2.40 |
CGCAACATACTGGAAACCCA | ||||||
SLC22A5 | GAACTCACGAGCCTCGCACGC | NM_019269 | 117 bp | -3.75 | 0.997 | 1.85 |
TCGTCGTAGTCCCGCATGCC | ||||||
SLC25A20 | AGCCCACCTGTTATCCACTG | NM_053965 | 178 bp | -3.32 | 0.988 | 2.00 |
TGTGCAAAAAGAGCCTTCCT | ||||||
SLC27A1 | GTATCTGCTGGACCTTCGC | NM_053580 | 243 bp | -3.48 | 0.990 | 1.94 |
CATAAATGAGGGCCTTGGCA | ||||||
TOP1 | GAAGAACGCTATCCAGAAGG | NM_022615 | 137 bp | -3.33 | 0.997 | 2.00 |
GCTTTGGGACTCAGCTTCAT | ||||||
UCP1 | CAGGCTTCCAGTACTATTAGG | NM_012682 | 181 bp | -3.40 | 0.983 | 1.97 |
CTCTCCCTGAAGAGAAGTACT | ||||||
UCP2 | CAAGGAGAGAGTCAAGGGCTA | NM_019354 | 209 bp | -3.08 | 0.998 | 2.11 |
GACTCTGAGCCCTTGGTGTAG | ||||||
UCP3 | CTCGGTACCATCCTGACTAT | NM_013167 | 149 bp | -3.47 | 0.982 | 1.94 |
GTTCCTTTGGGGGTGTAGAA | ||||||
VEGFA | GTTCATGGACGTCTACCAGC | NM_031836 | 253 bp | -3.62 | 0.973 | 1.89 |
GCTATGCTGCAGGAAGCTCA | ||||||
VEGFB | GTGTCCCAGTTTGATGGCC | NM_053549 | 187 bp | -3.45 | 1.000 | 1.95 |
CGTCAGGACAGCAGCCAC | ||||||
YWHAZ | GACGGAAGGTGCTGAGAAA | NM_013011 | 198 bp | -3.13 | 0.986 | 2.09 |
GCAGCAACCTCAGCCAAGT |