Effect of GSPs on islet cell apoptosis in normal and diabetic rats. The slides were visualized by fluorescence microscope. The insulin antibody and fluorescein isothiocyanate (FITC)-labeled secondary antibody were used to probe insulin (green). 4’, 6-diamidino-2-phenylindole (DAPI) was used to visualize nuclei (blue). Apoptotic cells exhibited strong nuclear red fluorescence using regional terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) staining. See Figure 2 for groups and treatment. Scale bar = 50 μm.