Figure 3

Increasing base pairings at either ends of the supplementary site has no effect on miR-30c and MTTP 3΄-UTR. (A) Base pairings between WT and different MTTP mutants and miR-30c are shown. Top row shows interactions between WT MTTP 3΄-UTR and miR-30c. The bottom two rows show mutations (shown as green alphabets) introduced in the MTTP sequence and their consequences on interactions with miR-30c. (B) Cos-7 cells were transfected with Scr or miR-30c as described in Methods and Figure 2. Subsequently, cells were transferred to other wells and transfected with luciferase expressing plasmids harboring either WT MTTP 3΄-UTR or different mutants described in Panel A. Significant differences between Scr and miR-30c are shown. Mean ± SD, n = 3. *p < 0.05, **p < 0.01.