Skip to main content
Figure 4 | Nutrition & Metabolism

Figure 4

From: Supplementary site interactions are critical for the regulation of microsomal triglyceride transfer protein by microRNA-30c

Figure 4

Increasing base pairing at the seed sequence and in the intervening loop enhances affinity between miR-30c and MTTP 3΄-UTR. (A) Interactions between WT and different MTTP 3΄-UTR mutants and miR-30c are shown. (B) Cos-7 cells were transfected with 50 nM of Scr or miR-30c as described in Figure 2 and Materials and Methods. Cells were then plated in different wells and transfected with plasmids expressing luciferase and containing either WT or mutant MTTP 3΄-UTR. After 17 h, cells were used to measure luciferase activities. Significant differences between Scr and miR-30c are shown. Mean ± SD, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page