Effect of different concentrations of mutants on their susceptibility to miR-30c. Cells were first transfected with different amounts of miR-30c. For control, cells were transfected with 100 nM Scr. Subsequently, cells were transfected with 1 μg of different indicated plasmids. Comparisons were made at each miR-30c concentrations with WT MTTP. Mean ± SD, n = 3. ##, P < 0.01.