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Figure 4 | Nutrition & Metabolism

Figure 4

From: Coffee polyphenols exert hypocholesterolemic effects in zebrafish fed a high-cholesterol diet

Figure 4

Effect of CPPs on vascular cholesterol accumulation in larval zebrafish. Larval zebrafish at 5 days post-fertilization were fed a control diet, a high-cholesterol diet, or a CPP diet; all diets additionally contained 0.002% (w/w) of the fluorescent cholesteryl ester BODIPY 576/589 C11. Diets were administered over 10 days. A, B: Optical (A) and fluorescent (B) images taken of zebrafish larvae at the end of the experiment. Arrows in these images identify the caudal artery. Scale bars: 200 μm. C: Fluorescence images of zebrafish larvae from the trunk to the tail, including the caudal artery (outlined by rectangles in Figure 4A and B), taken after 10 days of consumption of a diet supplemented with fluorescent cholesterol. Scale bars: 200 μm. D: Fluorescence intensities of the caudal arteries of fish from the three dietary groups (n = 10). Values are means ± SE. ##P < 0.01 vs. fish fed a control diet; **P < 0.01 vs. fish fed a high-cholesterol diet.

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