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Table 4 Effect of HCD in P2Y 13 -/- mice on hepatic mRNA expression of genes involved in lipid homeostasis

From: Lack of P2Y13 in mice fed a high cholesterol diet results in decreased hepatic cholesterol content, biliary lipid secretion and reverse cholesterol transport

Gene   Fold-change Accession N° Gene title
Scarb1 1.00 ± 0.12 0.86 ± 0.10 (p = 0.67) NM_016741 Scavenger receptor class B, member 1
Ldlr 1.00 ± 0.17 1.02 ± 0.15 (p = 0.75) NM_010700 Low density lipoprotein receptor
Abca1 1.00 ± 0.16 0.59 ± 0.05* (p = 0.04) NM_013454 ATP-binding cassette, sub-family A, member 1
Abcg1 1.00 ± 0.10 0.68 ± 0.07* (p = 0.02) NM_009593 ATP-binding cassette, sub-family G, member 1
Apoa1 1.00 ± 0.10 0.97 ± 0.07 (p = 0.72) NM_009692 Apolipoprotein A-I
Cyp7a1 1.00 ± 0.23 1.61 ± 0.24 (p = 0.06) NM_007824 Cytochrome P450, family 27, subfamily A, polypeptide 1
Cyp27a1 1.00 ± 0.13 1.11 ± 0.13 (p = 0.53) NM_024264 Cytochrome P450, family 8, subfamily B, polypeptide 1
Cyp8b1 1.00 ± 0.22 1.64 ± 0.26 (p = 0.18) NM_010012 Cytochrome P450, family 8, subfamily B, polypeptide 1
Abcg5 1.00 ± 0.12 0.64 ± 0.05* (p = 0.03) NM_031884 ATP-binding cassette, sub-family G, member 5
Abcg8 1.00 ± 0.10 0.61 ± 0.07** (p = 0.003) NM_026180 ATP-binding cassette, sub-family G, member 8
Abcb4 1.00 ± 0.07 0.74 ± 0.08 (p = 0.06) NM_008830 ATP-binding cassette, sub-family G, member 4
Abcb11/Bsep 1.00 ± 0.11 1.33 ± 0.10 (p = 0.22) NM_021022 ATP-binding cassette, sub-family B (MDR/TAP), member 11
Ntcp/Slc10a1 1.00± 0.22 1.51 ± 0.17 (p = 0.11) NM_011387 Solute carrier family 10 (sodium/bile acid cotransporter family), member 1
Oatp/Slco1a1 1.00 ± 0.16 1.33 ± 0.19 (p = 0.47) NM_013797 Solute carrier organic anion transporter family, member 1A2
Hmgcr 1.00 ± 0.01 1.02 ± 0.09 (p = 0.44) NM_008255 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Srebp2 1.00 ± 0.09 0.74 ± 0.07 (p = 0.06) NM_033218 Sterol regulatory element binding transcription factor 2
  1. Real-time PCR was performed on individual livers of 3 h fasted mice (n = 10 mice per group). For all genes, the fold change was calculated by dividing the P2Y13-/- mice value by the wild-type mice value (e.g. an increase of 80% from wild-type is reported as 1.80). * and ** indicate significant difference (p < 0.05 and p < 0.01 respectively) from wild-type mice.