Western blot analysis of NF-κB p65, AP-1, Nrf2 (nuclear fraction) and HO-1 (cytosolic fraction) in kidney cells in rats: Western blot using the anti- NF-κB (Panel A), AP-1 (Panel B), Nrf2 (Panel C) and hemeoxygenese-1 (HO-1; Panel D) revealed specific bands. Blots were repeated at least 3 times. β-actin levels were monitored to ensure equal protein loading (bottom panel). The intensity of the bands was quantified by the densitometric analysis. Data are percent of the control. a-c: Means in the same line without a common superscript differ significantly (P < 0.05).