Immunohistochemical localization of TLR4 and TRAF6 in jejunal and ileal tissues. Magnification is 200. TLR4 and TRAF 6 were expressed on intraepithelial lymphocytes and lymphocytes in submucosa. In addition weak positive staining was observed in the crypt region. MTX-induced damage was associated with decreased TLR4 and TRAF6 staining. The number of TLR4 and TRAF6 positive cells increased significantly following glutamine administration. CONTR-control, MTX-methotrexate, GLN-glutamine. *P < 0.05 MTX and MTX-GLN vs CONTR rats. †P < 0.05 MTX-GLN vs MTX rats.