Skip to main content
Figure 1 | Nutrition & Metabolism

Figure 1

From: A diet containing a nonfat dry milk matrix significantly alters systemic oxylipins and the endocannabinoid 2-arachidonoylglycerol (2-AG) in diet-induced obese mice

Figure 1

Discriminant analysis clearly segregates dietary treatment groups in a mouse model of developing obesity (Cohort 1). A PLS-DA model using physiological measurements, 61 plasma lipid metabolites, and mRNA relative abundance in retroperitoneal adipose tissue (A-), liver (L-), and nodose ganglia (N-) illustrates differential distribution between DIO mice fed 0.5% calcium (Control; pink), 1.5% calcium (HighCa; yellow), or 1.5% calcium + nonfat dry milk (NFDM; green). A plots the latent variable 1 (LV1; X axis) and 2 (LV2; Y axis) scores for each mouse, while B plots the variable weights in these axes. Variables were grouped and color coded based on a hierarchical cluster analysis. Selected variables in clusters and lipid classes driving group segregation are labeled (for a complete list of variables, see Additional file 3: Tables S1 and S2). Plasma fatty acid diols cluster with Adiposity Index and 18 carbon acylethanolamides (green cluster) and are reduced in NFDM-fed mice relative to Control and HighCa fed mice, along LV1. Conversely, monoacylglycerols cluster with expression of soluble epoxide hydrolase and adipose triglyceride lipase in the liver, and are elevated in NFDM-fed mice. Body weight, adiposity index, energy intake, and mRNA abundance of retroperitoneal adipose tissue inflammatory markers (purple cluster) drive the separation of HighCa fed mice from NFDM mice and controls in LV2.

Back to article page