Table 1 List of primers used to amplify the desired genes
Primer name | 5′——————— < 3′ | Primer length | |
|---|---|---|---|
IL-2 | Forward | CCTGAGCAGGGAGAATTACA | 20 |
Reverse | TCCAGAACATGCCGCAGA | 18 | |
Fas | Forward | GAGAATTGCTGAAGACATGACAATCC | 26 |
Reverse | ATGGCTGGAACTGAGGTAGTTTTCAC | 26 | |
Akt1 | Forward | CCTTTATTGGCTACAAGGAACGG | 23 |
Reverse | GAAGGTGCGCTCAATGACTG | 20 | |
CD28 | Forward | GTATTCCTACAACCTTCTCGCAA | 23 |
Reverse | GGGGCTGATAGGTAAAATTCCCA | 23 | |
Cdc42 | Forward | TTCTGGTTGTGTTTCAACTGCT | 22 |
Reverse | CCTCCCTTGGACTGCATCTG | 20 | |
Inf- γ | Forward | GCCCAATATCTCGGATGCTTC | 21 |
Reverse | GCCAAAATAGCTTCGGTAATCCT | 23 | |
TNF-α | Forward | CCAACATGCTGATTGATGACACC | 23 |
Reverse | GAGAATGCCAATTTTGATTGCCA | 23 | |