Effects of dietary Res supplementation on hepatic steatosis in KKAy mice. (A) Gross specimens of livers from mice in each group are shown. (B) Liver biopsy evaluation. Frozen sections of liver tissue were produced, the fat was stained with Oil Red O, and the nuclei were stained with hematoxylin. The original magnification was × 200. (C) The degree of hepatic steatosis of the mice in the different groups by Oil Red O quantitative analysis. (D, E) Hepatic levels of TG and TC in mice were measured. Data are shown as the mean ± S.D. ##P < 0.01vs. C57BL/6 J. *P < 0.05 and **P < 0.01 vs. KKAy control. n = 10.