| Â | Diet |
|---|
| Â | CONT | CELL | FOS | GLUC | PECT |
|---|
Ingredients (% w/w) | Â | Â | Â | Â | Â |
  Maize starch | 46.57 | 41.57 | 41.57 | 41.57 | 41.57 |
  Maltodextrin | 15.5 | 15.5 | 15.5 | 15.5 | 15.5 |
  Sucrose | 10 | 10 | 10 | 10 | 10 |
  Casein | 14 | 14 | 14 | 14 | 14 |
  Soyabean oil | 4 | 4 | 4 | 4 | 4 |
  AIN-93 Mineral mix | 3.5 | 3.5 | 3.5 | 3.5 | 3.5 |
  AIN-93 Vitamin mix | 1 | 1 | 1 | 1 | 1 |
  Choline bitartrate | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
  L-cystine | 0.18 | 0.18 | 0.18 | 0.18 | 0.18 |
  Cellulose | 5 | 10 | 0 | 0 | 0 |
  FOS2 | 0 | 0 | 10 | 0 | 0 |
  Beta-glucan3 | 0 | 0 | 0 | 10 | 0 |
  Pectin4 | 0 | 0 | 0 | 0 | 10 |
ME5 (MJ/kg) | 15.7 | 15.2 | 15.2 | 15.2 | 15.2 |
GE6 (MJ/kg) | 17.2 | 17.4 | 17.2 | 17.1 | 17.1 |
Composition (% w/w) | Â | Â | Â | Â | Â |
  Dry matter | 91.9 | 92.2 | 92.2 | 91.9 | 92.0 |
  Total carbohydrate7 | 63.5 | 58.5 | 59.5 | 65.3 | 57.5 |
  Total nitrogen8 | 2.0 | 2.0 | 2.0 | 2.1 | 2.1 |
  Total fat9 | 4.5 | 5.0 | 5.2 | 4.7 | 4.7 |
  NSP10- soluble | 0.50 | 0.46 | 8.7811 | 7.7412 | 4.23 |
  NSP10- insoluble | 2.02 | 3.45 | 0.45 | 0.46 | 0.62 |
- 1All diets were based on AIN-93Â M diet (American Society for Nutrition, Bethesda, MD USA) and manufactured by Special Diet Services Ltd. (Witham, Essex UK).
- 2Fructo-oligosaccharide (Higher Nature FOS Powder; Revital Ltd., Ruislip, Middlesex UK).
- 3Oat beta-glucan (Cambridge Commodities Ltd., Ely, Cambridgeshire UK).
- 4Apple pectin (Solgar Apple Pectin; Revital Ltd.).
- 5ME, metabolisable energy calculated from Atwater Fuel Energy of diet components.
- 6GE, gross energy determined using Gallenkamp Adiabatic Bomb Calorimeter.
- 7Total carbohydrate by standard acid-hydrolysis followed by colorimetric glucose determination.
- 8Total nitrogen determined using VarioMax CN Analyser.
- 9Total fat measured using standard acid-hydrolysis and gas chromatography.
- 10NSP, non-starch polysaccharides, analysed by the Englyst procedure[27], using gas chromatography and spectrophotometry to measure constituent sugars, and including
- 11fructose detection for FOS diet by ultra performance liquid chromatography.
- 12GLUC diet analysed specifically for beta-glucan by assay kit (K-BGLU; Megazyme, Bray, Co. Wicklow, Ireland).
