Figure 4

Effect of vitamin A on epididymal WAT (eWAT) Bcl2 and Bax protein expressions in lean and obese rats of WNIN/Ob strain. A. i) Representative western blot showing the levels of Bcl2 and Bax in eWAT of lean rats & ii) Histogram represents eWAT Bcl2–Bax ratio quantified densitometric values expressed relative to a value of 1 for control diet-fed lean rats (AI). B. i) Representative western blot showing the levels of Bcl2 and Bax in eWAT of obese rats & ii) Histogram represents eWAT Bcl2–Bax ratio quantified densitometric values expressed relative to a value of 1 for control diet-fed obese rats (BI). Equal loading of the protein was ensured by staining the membranes with Ponceau S (image not shown). Bars are given as means ± SE of 4 rats. Vitamin A-enriched diet was compared to stock diet of respective phenotypes. P < 0.05 was considered significant (one-way ANOVA). AI, BI- control diet, AII, BII- 2.6 mg/kg diet, AIII, BIII- 52 mg/kg diet & AIV, BIV- 129 mg/kg diet fed groups. ^a-bBars not sharing a common superscript are significantly different by one-way ANOVA and LSD post hoc comparison (P < 0.05).