Bone marrow derived macrophage effector functions. Bone marrow cells were isolated from the bone marrow from mice fed on a low protein (MN, n =4) or normal (control, n =4) diet for 16 day and were differentiated in conditioning medium. Eight days later, the capacity of these cells to phagocytose particles was determined by flow cytometry (A). In addition, BMMΦ were activated with IL-4, IFN-γ and TNF-α, LPS or left unactivated (nil) and the production of nitric oxide (B) and the activity of arginase (C) were measured as described in material and methods. Data show the results of one representative experiment out of four independent experiments. *: p <0.05. Box = interquartile range and median; whiskers = range. nd = not detectable.