Figure 4

Hcy induced nuclear import of Bach1 and the export of Nrf2. The cytosolic and nuclear proteins were extracted separately after Hcy treatment and subjected to Western blotting assay. Representative images of three independent experiments were shown. The cytosolic (A) and nuclear (B) proteins were present to illustrate the changes of subcellular localization of Nrf2 and Bach1. The values of density of proteins were justified with β-actin (C) and Histone (D), accordingly. The relative density ratios of untreated cells were set at a value of 1.0. The values are means ± SEM from three independent experiments. *P < 0.05 vs. control.