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Table 2 Composition of the experimental diets‡

From: High-cholesterol diet enriched with onion affects endothelium-dependent relaxation and NADPH oxidase activity in mesenteric microvessels from Wistar rats

Ingredient (g/kg)

Control

HC

HCO

Onion powder

−

−

100

Casein

200

200

200

Sucrose

100

100

100

Maize starch

470.49

445.49

368.69

Soya oil

50

50

50

Maize oil

80

80

80

Mineral mixture*

35

35

35

Vitamin mixture†

10

10

10

Cellulose powder

50

50

26.8

Choline bitartrate

2.5

2.5

2.5

tert-butylhydroquinone

0.010

0.010

0.010

L-cystine

2

2

2

Cholesterol

−

20

20

Cholic acid

−

5

5

  1. HC, high-cholesterol diet; HCO, high-cholesterol enriched with onion diet.
  2. *Mineral mix for the AIN-93 M diet, g/kg: calcium carbonate anhydrous, 357.00; potassium phosphate monobasic, 250.00; potassium citrate, tripotassium monohydrate, 28.00; sodium chloride, 74.00; potassium sulphate, 46.00; magnesium oxide, 24.00; ferric citrate, 6.06; zinc carbonate, 1.65; sodium meta-silicate 9H2O, 1.45; manganous carbonate, 0.63; cupric carbonate, 0.30; chromium potassium sulfate 12H2O, 0.275; boric acid, 0.0815; sodium fluoride, 0.0635; nickel carbonate, 0.0318; lithium chloride, 0.0174; sodium selenate anhydrous, 0.01025; potassium iodate, 0.0100; ammonium paramolybdate 4H2O, 0.00795; ammonium vanadate, 0.0066; powdered sucrose, 209.806.
  3. †AIN-93-VX vitamin mix for the AIN-93 M diet, g/kg: niacin, 3.000; calcium pantothenate, 1.600; pyridoxine-HCl, 0.700; thiamin-HCl, 0.600; riboflavin, 0.600; folic acid, 0.200; biotin, 0.200; vitamin B12 (0.1%), 2.500; vitamin E (all-rac-α-tocopheryl acetate, 500 IU/g), 15.000; vitamin A (all-trans-retinyl palmitate, 500,000 IU/g), 0.800; vitamin D3 (400,000 IU/g), 0.250; vitamin K1, 0.075; powdered sucrose, 974.655.
  4. ‡Diet energy content was calculated using the factors 16.73 kJ/g (4 kcal/g) for protein, 15.69 kJ/g (3.75 kcal/g) for monosaccharides, 16.53 kJ/g (3.95 kcal/g) for disaccharides, 17.49 kJ/g (4.18 kcal/g) for starch, 8.37 kJ/g (2 kcal/g) for dietary fibre, and 37.65 kJ/g for fat. Control diet, 18540.9 kJ/kg (4431.4 kcal/kg); HC diet, 18856.6 kJ/kg (4506.8 kcal/kg); HCO diet, 18642.4 kJ/kg (4455.6 kcal/kg).