Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach

Nutrition & Metabolism

Table 2 Fold differences in abundance of hepatic proteins identified by LC-MS/MS in C57BL/6 mice fed a diet high or low in n-3 polyunsaturated fatty acids

#Protein	Function	High n-3 PUFA	Low n-3 PUFA	GI	Mass	Score	Matches
Ornithine aminotransferase	Nitrogen homeostasis	28.25↑		8393866	48723	60	2 (1)
S-adenosylmethionine synthase	One-carbon metabolism	1.88↑		19526790	44051	503	11 (8)
Disulfide isomerase-A3	Protein folding	9.18↑		351707448	57366	81	2 (1)
Aldehyde dehydrogenase	Energy production		1.43↑	560645	56686	55	1 (1)
Adenosine kinase	Phosphorylation		1.66↑	19527306	40466	379	12 (2)
Lactoylglutathione lyase	Detoxification	1.69↑		165932331	20967	278	7 (6)

The images of the total protein pattern of 2D-gels were captured by video imaging using the ImageScanner III (LabScan 6.0 software, GE Healthcare, Life Sciences, Sweden). Progenesis Samespots, version 3.1, a specialized software (Non-linear Dynamics, Newcastle upon Tyne, UK) was then used to align and quantify the protein spots from the captured 2D-gel images. PUFA, polyunsaturated fatty acids; GI, GenBank sequence identification number; ↑= upregulation.

ISSN: 1743-7075