Oxidative stress is ameliorated in diabetic rats receiving the supplementation. (a) Total ROS levels were quantified in the retina (5-10 μg) using 2 μM of DCHFDA and the fluorescence emitted was measured at 485 nm and 530 nm. (b) Total antioxidant capacity of the retina was quantified by measuring the ability of the retina to inhibit oxidation of ABTS by metmyoglobin using 5-10 μg retina protein. Each sample was measured in duplicate, and the values are represented as mean ± SD of 6–8 rats in each group. *p < 0.05 and #p < 0.05 compared to normal and diabetes respectively.