Reversal of High dietary fructose-induced PPARα suppression by oral administration of lipoxygenase/cyclooxygenase inhibitors

Table 1 Gene-specific primer used for Real Time RT-PCR assays

GenBank Accession Number	Primer Sequence (5'→3')	Amplicon Size (bp)	Region of Gene (nt)
NM_013196	Rat PPARα	177
	Forward TCACACAATGCAATCCGTTT		876–895
	Reverse GGCCTTGACCTTGTTCATGT		1052–1033
V01279	*Rat 18S rRNA*	133
	Forward ATGGCCGTTCTTAGTTGGTG		1336–1355
	Reverse AACGCCACTTGTCCCTCTAA		1468–1449

Primer sequences were designed using Primer Express software (Applied Biosystems) using sequences accessed through GenBank. Amplicon size and region of each gene are indicated.

ISSN: 1743-7075