Mass of Food Consumed during Timed Re-Feeding Periods Following Food Deprivation in the BALB/C mouse. Food deprivation for up to 6 h, and then re-fed for 60 min consumed the same amount of food compared to the ad libitum (control) animals. However, after 9 h of deprivation, mice consumed only 50% of food present even after 1 h of re-feeding, compared to the controls. Retired male BALB/C breeder mice (n = 3/group) were deprived of food (LabDiet Rodent Diet 5001), water and bedding for periods of 1, 3, 6, 9, 12, and 24 h. Subsequently, re-feeding for 5, 30 and 60 min duration was carried out and the mass of food consumed during these periods was determined and compared to the mass of food consumed by the ad libitum controls. The analysis of variance revealed a significant difference in the ad libitum Control [F(5,12) = 192.67, p < 0.001], 30 min refeeding [F(5,12) = 5.87, p < 0.006] and 60 min refeeding [F(5,12) = 16.40, p < 0.001] groups. No significance was found in the 5 min refeeding group upon deprivation [F(5,12) = 1.19, p = 0.370].