ACC Activity in Whole Brain during Food Deprivation. 3a (ACC, assayed without citrate). 3b (ACC, assayed with 0.05 mM citrate). The ACC activity without citrate initially decreased by 42% with no significant change between 1 h and 24 h of deprivation. In the presence of citrate, the ACC activity decreased gradually over 24 h with a significant 32% decrease at 24 h deprivation. ACC activity assayed with and without citrate of ad libitum (controls), 1 h deprivation, and 24 h deprivation groups are represented. Whole brain was dissected, homogenized and the extracts were assayed for ACC activity in the presence or absence of citrate. The ACC activity assay mixture contained 5.0 mM HEPES, 0.25 mM MnCl2, 0.2 mM DTT, 0.01235 mM acetyl CoA, 0.4 mM ATP, 0.075 mg/mL BSA, 2.51 mM NaHCO3- (containing 1.11 × 105 cpm/nmol) as described in detail in the methods section. Results are expressed as the mean ± SD of assays of the brain extracts from four independent animals. Each extract was assayed in duplicate. A one-way analysis of variance revealed significant differences in the presence of citrate between 1 h and 24 h deprivation [F(2,6) = 9.89, p < 0.012]. When citrate was absent, significant differences between groups were also present [F(2,9) = 7.24, p < 0.013], however, no statistical significant was shown specifically between the 1 h starvation and 24 h starvation groups.