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Table 1 Primer sequences and PCR parameters

From: Differential effects of curcumin on vasoactive factors in the diabetic rat heart

Gene

Primers (5' → 3')

Temperature*

Rat eNOS

GCAAGACCGATTACACGACA

GTCCTCAGGAGGTCTTGCAC

Denaturation

Annealing

Extension

Signal

95°C-0 sec

57°C-5 sec

72°C-10 sec

85°C-1 sec

Human eNOS [42]

CCTCCAGGAAGGAGCAAAC

TCCTGAGAGAGAGGCAAGAGGA

Denaturation

Annealing

Extension

Signal

95°C-0 sec

55°C-5 sec

72°C-8 sec

81°C-1 sec

Rat iNOS

ATGGAACAGTATAAGCGAAACACC

GTTTCCGGTCGATGTCATGAGCAAAGG

Denaturation

Annealing

Extension

Signal

95°C-0sec

57°C-5 sec

72°C-10 sec

83°C-1 sec

Human iNOS [42]

CCCCATCAAGCCCTTTACTT

CACCTCCTGGTGGTCACTT

Denaturation

Annealing

Extension

Signal

95°C-0 sec

55°C-5 sec

72°C-8 sec

81°C-1 sec

Rat ET-1

GCTCCTGCTCCTCCTTGATG

CTCGCTCTATGTAAGTCATGG

Denaturation

Annealing

Extension

Signal

95°C-0 sec

55°C-5 sec

72°C-20 sec

84°C-1 sec

Human ET-1

AAGCCCTCCAGAGAGCGTTAT

CCGAAGGTCTGTCACCAATGT

6FAM-TGACCCACAACCGAG-MGBNFQ

Denaturation

Annealing

Extension

Signal

95°C-0 sec

55°C-5 sec

72°C-4 sec

72°C-1 sec

Human HO-1 [43]

TGATAGAAGAGGCCAAGA

TTTCCAGACAGAGGGACA

Denaturation

Annealing

Extension

Signal

95°C-0 sec

50°C-10 sec

72°C-17 sec

84°C-1 sec

Human HO-2 [43]

TGGAGCGCAACAAGGACCAT

CCGGTAGAGCTGCTTGAACT

Denaturation

Annealing

Extension

Signal

95°C-0 sec

50°C-10 sec

72°C-17 sec

84°C-1 sec

β-actin

CCTCTATGCCAACACAGTGC

CATCGTACTCCTGCTTGCTG

Denaturation

Annealing

Extension

Signal

95°C-0 sec

58°C-5 sec

72°C-8 sec

83°C-1 sec

  1. * Initial denaturation was carried out at 95 °C for 3 minutes.