From: Differential effects of curcumin on vasoactive factors in the diabetic rat heart
Gene | Primers (5' → 3') | Temperature* | |
---|---|---|---|
Rat eNOS | GCAAGACCGATTACACGACA GTCCTCAGGAGGTCTTGCAC | Denaturation Annealing Extension Signal | 95°C-0 sec 57°C-5 sec 72°C-10 sec 85°C-1 sec |
Human eNOS [42] | CCTCCAGGAAGGAGCAAAC TCCTGAGAGAGAGGCAAGAGGA | Denaturation Annealing Extension Signal | 95°C-0 sec 55°C-5 sec 72°C-8 sec 81°C-1 sec |
Rat iNOS | ATGGAACAGTATAAGCGAAACACC GTTTCCGGTCGATGTCATGAGCAAAGG | Denaturation Annealing Extension Signal | 95°C-0sec 57°C-5 sec 72°C-10 sec 83°C-1 sec |
Human iNOS [42] | CCCCATCAAGCCCTTTACTT CACCTCCTGGTGGTCACTT | Denaturation Annealing Extension Signal | 95°C-0 sec 55°C-5 sec 72°C-8 sec 81°C-1 sec |
Rat ET-1 | GCTCCTGCTCCTCCTTGATG CTCGCTCTATGTAAGTCATGG | Denaturation Annealing Extension Signal | 95°C-0 sec 55°C-5 sec 72°C-20 sec 84°C-1 sec |
Human ET-1 | AAGCCCTCCAGAGAGCGTTAT CCGAAGGTCTGTCACCAATGT 6FAM-TGACCCACAACCGAG-MGBNFQ | Denaturation Annealing Extension Signal | 95°C-0 sec 55°C-5 sec 72°C-4 sec 72°C-1 sec |
Human HO-1 [43] | TGATAGAAGAGGCCAAGA TTTCCAGACAGAGGGACA | Denaturation Annealing Extension Signal | 95°C-0 sec 50°C-10 sec 72°C-17 sec 84°C-1 sec |
Human HO-2 [43] | TGGAGCGCAACAAGGACCAT CCGGTAGAGCTGCTTGAACT | Denaturation Annealing Extension Signal | 95°C-0 sec 50°C-10 sec 72°C-17 sec 84°C-1 sec |
β-actin | CCTCTATGCCAACACAGTGC CATCGTACTCCTGCTTGCTG | Denaturation Annealing Extension Signal | 95°C-0 sec 58°C-5 sec 72°C-8 sec 83°C-1 sec |