Figure 2

Effects of resistin on gene expression patterns during adipogenesis. Day 0 represents 3T3L1 fibroblasts reaching 100% confluence. Two days after full confluence (Day 2), cells were placed in DMEM containing 25 mM glucose, 0.5 mM isobutylmethylxanthine (IBMX), 1 μm dexamethasone (Dex), 10 μg/ml insulin, and 10% FBS for 3 days; then, on day 5 cells were placed in DMEM containing 25 mM glucose, 10 μg/ml insulin, and 10% FBS for 2 days. After day 7, cells were maintained in DMEM, 25 mM glucose, and 10% FBS. At the indicated time points, resistin and LacZ transduced preadipocytes and adipocytes were lysed and the mRNA levels encoding PPARγ, C/EBPα, and ALBP/aP2 were quantified by quantitative PCR. Results represent the mean ± SE from three separate experiments. Asterisk, P < 0.05; double asterisk, P < 0.01 for resistin versus control cells.