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Table 2 Dependence of binding kinetics on pH and ionic strength as measured by SPR

From: Characterization of a monoclonal antibody with specificity for holo-transcobalamin

Immobilized reagent pH NaCl (mmol/L) kon (104 M-1s-1) koff (10-4 s-1) KD (nmol/L)
HoloTC 6.0 0 10 1 1
  7.4 0 8 0.4 0.5
  7.4 300 7 0.6 0.9
  8.0 0 7 0.2 0.3
MAb 3C4 6.0 0 50 4 0.8
  6.0 300 0.3 35 1340
  7.4 0 3 14 46
  7.4 300 0.3 41 1380
  8.0 0 7 19 27