Table 2 Dependence of binding kinetics on pH and ionic strength as measured by SPR
From: Characterization of a monoclonal antibody with specificity for holo-transcobalamin
Immobilized reagent | pH | NaCl (mmol/L) | kon (104 M-1s-1) | koff (10-4 s-1) | KD (nmol/L) |
|---|---|---|---|---|---|
HoloTC | 6.0 | 0 | 10 | 1 | 1 |
| Â | 7.4 | 0 | 8 | 0.4 | 0.5 |
| Â | 7.4 | 300 | 7 | 0.6 | 0.9 |
| Â | 8.0 | 0 | 7 | 0.2 | 0.3 |
MAb 3C4 | 6.0 | 0 | 50 | 4 | 0.8 |
| Â | 6.0 | 300 | 0.3 | 35 | 1340 |
| Â | 7.4 | 0 | 3 | 14 | 46 |
| Â | 7.4 | 300 | 0.3 | 41 | 1380 |
| Â | 8.0 | 0 | 7 | 19 | 27 |