Figure 1
From: Modulation of adipocyte lipogenesis by octanoate: involvement of reactive oxygen species
The effects of octanoate on lipogenesis in adipocytes. Six days after induction of differentiation, 3T3-L1 adipocytes were incubated with octanoate (1 mM, oct) mixed with DMEM containing 10% FBS and 170 nM insulin for 3 days. Exogenous octanoate were then removed and cells were incubated for 3 h in the same medium with 3H2O (A), [9,10-3H] triolein (B), and [U-3H] glucose (C). The incorporation of the corresponding isotope into the cellular TAG-fatty acids (TAG-FA, A&B) and TAG-glycerol (C) were measured. Data are mean +/- SE, n = 3. *p < 0.05 compared to control.