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Figure 3 | Nutrition & Metabolism

Figure 3

From: Modulation of adipocyte lipogenesis by octanoate: involvement of reactive oxygen species

Figure 3

The regulation of β-oxidation of octanoate (A) and oleate (B) in adipocytes. Cells were serum-starved overnight in DMEM containing 0.5% BSA. (A) Cells were then incubated in DMEM for 2 h with exogenous octanoate [0.5 mM, 1 μCi/ml)] alone or together with insulin (170 nM, ins), etomoxir (30 μM, etx), L-carnitine (5 mM, L-car), or L-carnitine plus oleate (0.5 mM, L-car + ole). The release of 14CO2 was measured. (B) Cells were pre-incubated with [9,10-3H] oleate overnight. After washing off the exogenous oleate, cells were incubated with L-carnitine (5 mM) or insulin (170 nM) in DMEM for 2 h. The release of 3H2O into the medium was measured. Results are mean +/- SE. Values indicated with different letters are significantly different (p < 0.05).

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