Figure 4From: Modulation of adipocyte lipogenesis by octanoate: involvement of reactive oxygen speciesThe effects of octanoate on the generation of reactive oxygen species (ROS, A), PPARγ transcription activity (B) and TAG synthesis (C), and the effects of antioxidants. For (A), 3T3-L1 adipocytes were incubated with octanoate (1 mM) for 24 h with or without N-acetylcysteine (NAC, 20 mM) followed with the measurement of ROS by the DCF assay. Results are representative of at least three independent experiments. For (B), 293A-PPARγ2 cells were co-transfected with the plasmid DNA vector encoding a PPRE(+)-Luc reporter gene or the Rluc control reporter gene. After 24 h, cells incubated with octanoate (1 mM) or a mixture of xanthine/xanthine oxidase (200 μM/30 μU/ml). Cells were harvested after 24 h and analyzed using a dual luciferase assay. For (C), 3T3-L1 adipocytes were infected with recombinant adenovirus encoding GPx1 or its parental virus Ad-5. After 24 h, Cells were incubated with octanoate (1 mM) for another 3 days. TAG synthesis from [U-3H] glucose was measured as described in Figure 1. For (B&C), Results are mean +/- SE, n = 3, *p < 0.05 compared to control.Back to article page