Effect of AICAR on glucose and insulin tolerance tests in AICAR treated STD and DIO treated mice. For glucose tolerance test, overnight-fasted mice from all groups were given an intraperitoneal injection of glucose (2 mg/g body wt). Blood samples were withdrawn from the tail and analyzed for glucose levels at the indicated time points (15 to 120 min) (a). For insulin tolerance test, insulin (0.5 units/kg body wt) was intraperitoneal injected after fasting for 12 h, and glucose levels were monitored at the indicated time points (15 to 120 min)(b). Results are the means ± SD from four animals.