Effect of AICAR on phospho-status of ACC, AMPK during adipocyte differentiation. The differentiating 3T3-L1 cells were treated with different concentrations of AICAR (from 0.1 to 1.0 mM) and proteins were isolated at various time periods (from 0 to 9 days). Immunoblot analysis for p-ACC, p-AMPKα, AMPKα1, AMPKα2 and β actin was examined as described in Materials and Methods (a). 3T3L1 cells were differentiated as above in the presence or absence of AICAR (0.5 and 1.0 mM)/iodotubercidin (0.1 μM) for 9 days. Cells were processed for the immunoblot analysis for p-ACC and β actin (b).