AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic alterations in diet-induced obesity mice model

Table 1 Effects of AICAR treatment on fatty acid composition (weight %) of adipose tissue in normal and diet-induced obese mice

Group/Fatty acid	14:0	16:0	18:0	18:2
STD	0.89 ± 0.02	18.6 ± 0.1	1.9 ± 0.06	27.1 ± 0.35
STD+A500	0.9 ± 0.01	22.2 ± 0.21	3.0 ± 0.15	28.9 ± 0.81
DIO	1.3 ± 0.2	26.6 ± 0.21*	3.6 ± 0.15*	15.9 ± 1.3*
DIO+A100	0.95 ± 0.03	24.3 ± 0.38	4.4 ± 0.06	28.7 ± 0.86
DIO+A500	1.1 ± 0.1	23.3 ± 0.26**	2.5 ± 0.21**	22.2 ± 0.7**

Adipose tissue was isolated and prepared as described in Methods. The lipids were extracted in chloroform-methanol using Folch partition. Fatty acid methyl ester was prepared with methanolic-HCl, purified by TLC and analyzed by GC as described in our earlier publication [29]. Fatty acids showing significant alteration in composition are included and their peaks were identified using known standards. Values are expressed as mean ± SD for 3 separate sets of experiments. *p < 0.001 vs STD, **p < 0.001 vs DIO.

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