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Table 1 Effects of AICAR treatment on fatty acid composition (weight %) of adipose tissue in normal and diet-induced obese mice

From: AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic alterations in diet-induced obesity mice model

Group/Fatty acid 14:0 16:0 18:0 18:2
STD 0.89 ± 0.02 18.6 ± 0.1 1.9 ± 0.06 27.1 ± 0.35
STD+A500 0.9 ± 0.01 22.2 ± 0.21 3.0 ± 0.15 28.9 ± 0.81
DIO 1.3 ± 0.2 26.6 ± 0.21* 3.6 ± 0.15* 15.9 ± 1.3*
DIO+A100 0.95 ± 0.03 24.3 ± 0.38 4.4 ± 0.06 28.7 ± 0.86
DIO+A500 1.1 ± 0.1 23.3 ± 0.26** 2.5 ± 0.21** 22.2 ± 0.7**
  1. Adipose tissue was isolated and prepared as described in Methods. The lipids were extracted in chloroform-methanol using Folch partition. Fatty acid methyl ester was prepared with methanolic-HCl, purified by TLC and analyzed by GC as described in our earlier publication [29]. Fatty acids showing significant alteration in composition are included and their peaks were identified using known standards. Values are expressed as mean ± SD for 3 separate sets of experiments. *p < 0.001 vs STD, **p < 0.001 vs DIO.