Western Blot analysis of SR-BI protein. Cell lysates were prepared from control and Bt2cAMP (2.5 mM)-treated Y1-BS1 adrenal cells. Suitable aliquots (10–40 μg protein) were subjected to SDS-PAGE followed by transfer of proteins from gel to Immobiolon® membranes. The blots were incubated with rabbit anti-SR-BI and developed with an HRP-based chemiluminescent detection system. Arrows indicate the position of SR-BI monomer and dimer/oligomers. The approximate molecular weights of top and bottom bands are 160 kDa and 80 kDa, respectively.