A – Low magnification photo of a section through a highly expressing SR-BI-V5 transfected Y1-BS1 cell immunostained for the V5 tag. In addition to the totally disrupted cell surface, note the two complicated crater-like structures (see boxed areas) lined with convoluted double membrane channel structures. B – A higher magnification photo of the larger crater seen in figure 4A. Note the center of the mass is gone and just the coiled double membranes (arrows) representing the wall of the crater remain. One loop of coiled membrane opens to the cell surface (asterisk). The gold particles, representing SR-BI-V5 staining, are associated with vesicles and membranes of the crater structure.