Consequences of over-expression of rat Scavenger Receptor, SR-BI, in an adrenal cell model

Nutrition & Metabolism

Table 2 Selective HDL-CE uptake by control Y1-BS1 cells and cells transiently overexpressing SR-BI.

Experimental Conditions	Mass of selective HDL-CE uptake (ng CE/mg cell protein/5h ± SE)
Control (vector) transfected cells
• Basal	607 ± 128
• Bt₂cAMP (2.5 mM)	1802 ± 463^a
SR-BI transfected cells
• Basal	1623 ± 295^b
• Bt₂cAMP (2.5 mM)	3514 ± 48^c,d

Results are Mean ± SE of four separate experiments.
Y1-BS1 mouse adrenocortical cells were transfected with rSR-BI-V5-pcDNA6.1 construct (or control vector) for 48 h. Twenty four hours after transfection, some cultures were treated with Bt₂cAMP (2.5 mM) for an additional 24 h. Subsequently, cells were incubated with radiolabeled, non-releasable apolipoprotein ([¹²⁵I] DLT) and cholesteryl ester ([³H] COE) tags ± Bt₂AMP (2.5 mM) that would accumulate within cells. Uptake by the selective pathway was determined as described under Experimental Procedures.
^ap = 0.0471 vs basal control transfected cells; ^bp = 0.0196 vs basal control transfected cells; ^cp = 0.0159 vs basal SR-BI transfected cells; ^dp = 0.0434 vs Bt₂cAMP-treated control transfected cells.

ISSN: 1743-7075