Figure 3

siRNA-mediated reduction in LPL expression over a 5 day adipocyte differentiation period. 3T3-L1 cells were transfected with siRNA specific for LPL, followed by incubation with adipocyte induction reagents. After each 24 h period up to day 5 post-induction, total RNA was isolated from adipocytes and LPL expression was assessed by qRT-PCR. Results were analyzed by the Comparative C_T method with fully differentiated, non-treated adipocytes representing 100% LPL expression. β-actin expression was amplified in parallel and used to normalize replicate points.