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Table 4 Comparison of results obtained using a delta-delta (after correction using constitutive genes) method, quantitative RT-PCR approach (individual cDNA standard curves) and our postulated method (common cDNA bp-based standard curve) on liver and WAT from animals with restricted access to food and their controls.

From: Semiquantitative RT-PCR measurement of gene expression in rat tissues including a correction for varying cell size and number

ORGAN and gene Group Delta-delta Individual RT-PCR Present method
LIVER
Carbohydrate-responsive element-binding protein Control 100 ± 4 100 ± 5 100 ± 5
  Restricted 76 ± 4 79 ± 4 79 ± 4
Acetyl-CoA carboxylase 1 Control 100 ± 8 100 ± 3 100 ± 3
  Restricted 50 ± 12 47 ± 21 46 ± 1
Insulin receptor substrate 1 Control 100 ± 8 100 ± 9 100 ± 8
  Restricted 116 ± 10 115 ± 10 115 ± 10
ADIPOSE TISSUE
Carbohydrate-responsive element-binding protein Control 100 ± 12 100 ± 10 100 ± 9
  Restricted 24 ± 4 27 ± 5 27 ± 5
Type II iodothyronine deiodinase Control 100 ± 6 100 ± 6 100 ± 5
  Restricted 45 ± 3 46 ± 2 46 ± 2
Acetyl-CoA carboxylase 1 Control 100 ± 8 100 ± 13 100 ± 11
  Restricted 55 ± 14 53 ± 14 53 ± 11
Insulin receptor substrate 1 Control 100 ± 13 100 ± 12 100 ± 11
  Restricted 28 ± 5 29 ± 5 28 ± 4
  1. The data are the mean ± sem of 7 different animals and are expressed in all the cases as the percentage of the mean controls' values for easier comparison between the methods using homologous units. The base data used for comparisons in the case of the postulated method (and the individual quantitative RT-PCR method) was fmol/unit of total tissue RNA weight.