Figure 5From: Long term highly saturated fat diet does not induce NASH in Wistar ratsLiver capacity to oxidize fatty acids was assessed by (A) ketone body production and (B) oxygen uptake. Hepatocytes were isolated from 24 h starved Wistar rats. Cell viability was checked by a measure of (C) gluconeogenesis, in the presence of dihydroxyacetone and medium (octanoate) or long (oleate) chain fatty acids. (D) Rat liver mitochondria control state respiration was obtained with 5 Mm succinate/0.5 mM malate/1.25 μM rotenone as substrates. State 3 respirations were initiated with 1 mM ADP. Each determination was performed in triplicate from at least ten preparations for the three different diets: standard diet (white), coconut diet (black), butter diet (hatched). Results are means ± SEM. No significant difference between diets was observed.Back to article page