The effects of BSA, PA or OA on PPARα mRNA (A) and protein expression (B). Groups of islets isolated from KO and/or WT mice were exposed to control media (BSA, open bars), PA (closed bars) or OA (dashed bars) for 24 h. Treatment of WT and KO islets with PA and OA significantly increased PPARα mRNA expression compared to BSA (*P < 0.05). PPARα protein expression was significantly increased only in KO islets treated with OA (*P < 0.05). Two-way ANOVA performed on PPARα protein expression in KO and WT islets showed a significant difference between two genotypes (P < 0.05). Data are presented as means ± SE, N = 4–6 for each group.