TNFα treatment promotes degradation of IκB in adipocytes. (A) Adipocytes were incubated without (left panels) or with (right panels) TNFα for the indicated times. Total cellular proteins were solubilized and subject to immunoblot analysis using anti-IκB monoclonal antibody (upper panels). Samples were also immunoblotted with anti-β-actin monoclonal antibodies in parallel as a loading control (lower panels). (B) Densitometric quantitation of IκB shown in (A).