Flow cytometry analysis. (A) Live gate on leukocyte DNA (M1) during acquisition: Discrimination of leukocytes from debris, erythrocytes, platelets and bacteria. (B) Neutrophils and monocytes were identified by setting a polygonal gate in a forward scatter/sideward scatter dot plot. The negative control sample (C) was used to define a marker for rhodamine 123 (FL 1) where less than 5% of the cells would be positive. The percentage of neutrophils having produced hydrogen peroxide following lipid incubation was determined by counting the number of rhodamine positive cells above this marker position and by dividing it by the whole number of events observed (D).