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Figure 2 | Nutrition & Metabolism

Figure 2

From: Leucine modulation of mitochondrial mass and oxygen consumption in skeletal muscle cells and adipocytes

Figure 2

The effects of leucine, calcitriol and nifedipine on mitochondrial mass as assessed by NAO dye-binding as described in material and methods section in C2C12 myocytes treated with or without conditioned medium (CM) derived from differentiated adipocytes or co-cultured with differentiated 3T3-L1 adipocytes. The upper panel (A) shows fluorescent images of mitochondrial density and the lower panel shows the quantitative data in response to the treatments. Values are presented as mean ± SD, n = 6. Means with * differ with control(p < 0.005), ** differ with leucine (p = 0.01), *** differ with calcitriol (p < 0.001).

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