Figure 5
The effect of leucine, and/or nifedipine and/or calcitriol on mass by NAO binding in differentiated 3T3-L1 adipocytes. The upper panel (A) shows fluorescent images of mitochondrial density and the lower panel (B) shows the quantity data in response to the treatments. Cells were treated with or without leucine (0.5 mM), nifedipine (10 μM), or/and calcitriol (10 nM) for 48 hours. Values are presented as mean ± SD, n = 6. Means with * differ compared with control group with p < 0.04. ** differ with leucine (p < 0.05), *** differ with calcitriol (p < 0.05)